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omniscript rt kit  (Qiagen)


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    Structured Review

    Qiagen omniscript rt kit
    Omniscript Rt Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 98/100, based on 7875 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/omniscript+rt+kit/Omniscript+RT+Kit/pmc13158619-66-23-26
    Average 98 stars, based on 7875 article reviews
    omniscript rt kit - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Estrogen-related receptor gamma is required for normal auditory innervation and is essential for hearing
    Article Snippet: .. RNA was prepared from P1 cochlear tissue as already described and 900ng was used in cDNA synthesis with the Omniscript® RT Kit (QIAGEN®, #205113) supplemented with random primers (Promega, #C1181), and RNaseOutTM Ribonuclease Inhibitor (Invitrogen, #10777019). .. Real-Time quantitative PCR was performed with TaqManTM gene expression assays Esrrg: Mm00516267_m1, Gria2: Mm00442822_m1, Grip2: Mm01183453_m1, Kcnj3: Mm00434618_m1, Lypd1: Mm00513929_m1, Myo7a: Mm01274015_m1, Olig1: Mm00497537_s1, Slc6a11 : Mm00556476_m1, Slc17a7: Mm00812886_m1, Spock3: Mm00470030_m1, Syt7: Mm00444502_m1, and Tmem132d: Mm00556406_m1 with the SsoAdvanced Universal Probes Supermix (#1725281, Bio-Rad) on a Bio-Rad CFX ConnectTM Real-Time System. cDNA reactions (20ul) were diluted 1:3 and 1μl was used per qPCR reaction.

    Article Title: CRISPR-mediated functional mapping of IL2RG variants in primary human T cells predicts X-linked severe combined immunodeficiency
    Article Snippet: .. For cDNA preparation, cells were lysed and mRNA was purified using a Direct-zol RNA Miniprep Plus kit (Zymo Research) according to the manufacturer’s instructions and subsequently converted to cDNA using the Omniscript RT kit (Qiagen) or Maxima H Minus First Strand cDNA Synthesis kit with oligo(dT) (Thermo Fisher Scientific) according to the manufacturer’s instructions. ..

    Sequencing:

    Article Title: A SRC-Annexin A2 axis that couples membrane repair to microRNA export during radiation stress in glioblastoma
    Article Snippet: To quantify the absolute copy number of miR-603, cycle threshold (Ct) values were calibrated using a standard curve derived from a synthetic human miR-603 mimic (Qiagen, MSY0003271) serially diluted to a final concentration of 1×10 1 , 1×10 2 , 1×10 3 , 1×10 4 , 1×10 5 , 1×10 6 , 1×10 7 , and 1×10 8 copies. miRNA copies per EV were calculated by dividing the total EV numbers quantified by NanoSight. .. The full-length human ANXA2 coding sequence was amplified from cDNA generated using the Omniscript RT Kit (Qiagen) from total RNA isolated from LN340 cells. ..

    Amplification:

    Article Title: A SRC-Annexin A2 axis that couples membrane repair to microRNA export during radiation stress in glioblastoma
    Article Snippet: To quantify the absolute copy number of miR-603, cycle threshold (Ct) values were calibrated using a standard curve derived from a synthetic human miR-603 mimic (Qiagen, MSY0003271) serially diluted to a final concentration of 1×10 1 , 1×10 2 , 1×10 3 , 1×10 4 , 1×10 5 , 1×10 6 , 1×10 7 , and 1×10 8 copies. miRNA copies per EV were calculated by dividing the total EV numbers quantified by NanoSight. .. The full-length human ANXA2 coding sequence was amplified from cDNA generated using the Omniscript RT Kit (Qiagen) from total RNA isolated from LN340 cells. ..

    Generated:

    Article Title: A SRC-Annexin A2 axis that couples membrane repair to microRNA export during radiation stress in glioblastoma
    Article Snippet: To quantify the absolute copy number of miR-603, cycle threshold (Ct) values were calibrated using a standard curve derived from a synthetic human miR-603 mimic (Qiagen, MSY0003271) serially diluted to a final concentration of 1×10 1 , 1×10 2 , 1×10 3 , 1×10 4 , 1×10 5 , 1×10 6 , 1×10 7 , and 1×10 8 copies. miRNA copies per EV were calculated by dividing the total EV numbers quantified by NanoSight. .. The full-length human ANXA2 coding sequence was amplified from cDNA generated using the Omniscript RT Kit (Qiagen) from total RNA isolated from LN340 cells. ..

    Isolation:

    Article Title: A SRC-Annexin A2 axis that couples membrane repair to microRNA export during radiation stress in glioblastoma
    Article Snippet: To quantify the absolute copy number of miR-603, cycle threshold (Ct) values were calibrated using a standard curve derived from a synthetic human miR-603 mimic (Qiagen, MSY0003271) serially diluted to a final concentration of 1×10 1 , 1×10 2 , 1×10 3 , 1×10 4 , 1×10 5 , 1×10 6 , 1×10 7 , and 1×10 8 copies. miRNA copies per EV were calculated by dividing the total EV numbers quantified by NanoSight. .. The full-length human ANXA2 coding sequence was amplified from cDNA generated using the Omniscript RT Kit (Qiagen) from total RNA isolated from LN340 cells. ..

    Article Title: Combined Inhibition of ATR and Ribonucleotide Reductase Induces Synergistic Antineoplastic Activity in Osteosarcoma Cells.
    Article Snippet: .. Briefly, total RNA was isolated using the Peqgold Total RNA Kit including DNase digestion (Peqlab, Erlangen, Germany), RNA was transcribed into cDNA using the Omniscript RT Kit (Qiagen, Hilden, Germany), and realtime PCR was done on a Thermo Fisher Scientific Applied Biosystems 7900HT Real- Time PCR system. .. Reactions were carried out in duplicate using Applied Biosystems Gene Expression Assays and Universal PCR Master Mix.

    Reverse Transcription:

    Article Title: Distribution and molecular drivers of the 21-gene recurrence score in early breast cancer with low, intermediate, and high estrogen receptor expression
    Article Snippet: Subsequently, total RNA was extracted and purified from three 10-μm unstained sections using the RNeasy FFPE RNA kit (Qiagen, 73504, Germany). .. Gene-specific reverse transcription was conducted using Omniscript RT kit (Qiagen, 205111, Germany). .. Finally, quantitative RT-PCR was performed in 96-well plates with Applied Biosystems (Foster City, CA, USA) 7500 Real-Time PCR system.

    Article Title: Unraveling the role of ChREBP in lung adenocarcinoma: Expression, regulatory networks, and potential functional impact
    Article Snippet: RNA concentration and quality were assessed using a FLUOstar Omega instrument with an LVis Plate (BMG Labtech, Germany). .. Reverse transcription was performed to synthesize cDNA from the extracted RNA using Omniscript RT kit (Qiagen, Germany). ..

    Article Title: Development of a high-throughput screening platform for identification of functional BACH1 inhibitors reveals compounds with anti-invasive potential
    Article Snippet: .. RNA was extracted using GeneJET RNA Purification Kit (Thermo Fisher Scientific) and 500 ng of RNA per sample was reverse-transcribed to cDNA using Omniscript RT kit (Qiagen) supplemented with RNase inhibitor according to the manufacturer's instructions. .. The resulting cDNA was processed using TaqMan Universal Master Mix II (Life Technologies, Carlsbad, CA, USA) as well as corresponding Taqman probes.

    TaqMan Assay:

    Article Title: Optimization of an ex vivo model to test the ability of chemicals to disrupt thyroid hormone synthesis
    Article Snippet: .. In short, cDNA was prepared from 300 ng RNA using the Omniscript RT kit (Qiagen) according to the manufacturer's protocol. cDNA was diluted (1:20) for all TaqMan assays, except for cDNA used for the TaqMan assay Slc5a5 (NIS) (Rn00583900_m1), which was run separately with reference genes at a dilution of 1:6. .. The gene-specific TaqMan Gene Expression Assays (Life Technologies) were used: Rps18 (Rn01428913_g1) and Sdha (Rn00590475_m1) as reference genes, Tpo (Rn00571159_m1) and Slc5a5( NIS ) (Rn00583900_m1) due to their relation to TH synthesis and function, and Bcl-2 (Rn99999125_m1) and Bax (Rn01480161_g1) as apoptotic markers .

    Purification:

    Article Title: CRISPR-mediated functional mapping of IL2RG variants in primary human T cells predicts X-linked severe combined immunodeficiency
    Article Snippet: .. For cDNA preparation, cells were lysed and mRNA was purified using a Direct-zol RNA Miniprep Plus kit (Zymo Research) according to the manufacturer’s instructions and subsequently converted to cDNA using the Omniscript RT kit (Qiagen) or Maxima H Minus First Strand cDNA Synthesis kit with oligo(dT) (Thermo Fisher Scientific) according to the manufacturer’s instructions. ..

    Article Title: Development of a high-throughput screening platform for identification of functional BACH1 inhibitors reveals compounds with anti-invasive potential
    Article Snippet: .. RNA was extracted using GeneJET RNA Purification Kit (Thermo Fisher Scientific) and 500 ng of RNA per sample was reverse-transcribed to cDNA using Omniscript RT kit (Qiagen) supplemented with RNase inhibitor according to the manufacturer's instructions. .. The resulting cDNA was processed using TaqMan Universal Master Mix II (Life Technologies, Carlsbad, CA, USA) as well as corresponding Taqman probes.

    Polymerase Chain Reaction:

    Article Title: Combined Inhibition of ATR and Ribonucleotide Reductase Induces Synergistic Antineoplastic Activity in Osteosarcoma Cells.
    Article Snippet: .. Briefly, total RNA was isolated using the Peqgold Total RNA Kit including DNase digestion (Peqlab, Erlangen, Germany), RNA was transcribed into cDNA using the Omniscript RT Kit (Qiagen, Hilden, Germany), and realtime PCR was done on a Thermo Fisher Scientific Applied Biosystems 7900HT Real- Time PCR system. .. Reactions were carried out in duplicate using Applied Biosystems Gene Expression Assays and Universal PCR Master Mix.

    Real-time Polymerase Chain Reaction:

    Article Title: Combined Inhibition of ATR and Ribonucleotide Reductase Induces Synergistic Antineoplastic Activity in Osteosarcoma Cells.
    Article Snippet: .. Briefly, total RNA was isolated using the Peqgold Total RNA Kit including DNase digestion (Peqlab, Erlangen, Germany), RNA was transcribed into cDNA using the Omniscript RT Kit (Qiagen, Hilden, Germany), and realtime PCR was done on a Thermo Fisher Scientific Applied Biosystems 7900HT Real- Time PCR system. .. Reactions were carried out in duplicate using Applied Biosystems Gene Expression Assays and Universal PCR Master Mix.



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